Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.137 extracted from

  • Oeztuerk, B.; Ghequire, M.; Nguyen, T.P.; De Mot, R.; Wattiez, R.; Springael, D.
    Expanded insecticide catabolic activity gained by a single nucleotide substitution in a bacterial carbamate hydrolase gene (2016), Environ. Microbiol., 18, 4878-4887 .
    View publication on PubMed
Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Novosphingobium sp. KN65.2

Protein Variants

Protein Variants Comment Organism
L152F the mutation renders the enzyme completely inactive against carbofuran Novosphingobium sp. KN65.2

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Novosphingobium sp. KN65.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00053
-
 carbofuran at 37°C, pH not specified in the publication Novosphingobium sp. KN65.2

Organism

Organism UniProt Comment Textmining
Novosphingobium sp. KN65.2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Spin Protein Miniprep kit Novosphingobium sp. KN65.2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Novosphingobium sp. KN65.2 4-nitrophenol + acetate
-
 ?
aldicarb + H2O lowest activity Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?
carbaryl + H2O highest activity Novosphingobium sp. KN65.2 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O the extent of hydrolysis of carbofuran is about 0.6fold compared to carbaryl Novosphingobium sp. KN65.2 carbofuran phenol + methylamine + CO2
-
 ?
fenobucarb + H2O
-
 Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?
isoprocarb + H2O the extent of hydrolysis of carbofuran is about 0.5fold compared to carbaryl Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?
metolcarb + H2O
-
 Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?
oxamyl + H2O low activity Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?
propoxur + H2O
-
 Novosphingobium sp. KN65.2 ? + methylamine + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 88000, SDS-PAGE Novosphingobium sp. KN65.2
? x * 87600, calculated from amino acid sequence Novosphingobium sp. KN65.2

Synonyms

Synonyms Comment Organism
carbamate hydrolase
-
 Novosphingobium sp. KN65.2
cfdJ
-
 Novosphingobium sp. KN65.2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.46
-
 carbofuran at 37°C, pH not specified in the publication Novosphingobium sp. KN65.2