Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 60% inhibition in presence of Mg2+ | Pantoea agglomerans | |
dithiothreitol | 1 mM, 80% inhibition in presence of Mg2+ and 50% inhibition in absence of divalent cations | Pantoea agglomerans | |
Triton X-100 | 1%, 50% inhibition in absence of divalent cations | Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
(indol-3-yl)acetyl-L-aspartate | pH 7.0, 30°C | Pantoea agglomerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 40% increase in activity | Pantoea agglomerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | 55000 | gel filtration | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | O50173 | - |
- |
Purification (Comment) | Organism |
---|---|
partial | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)acetyl-L-aspartate + H2O | - |
Pantoea agglomerans | (indol-3-yl)acetate + L-aspartate | - |
? | |
additional information | enzyme is highly specific for (indol-3-yl)acetyl-L-aspartate. No substrates: indole-3-acetic acid, ammonium sulfate, urea, indoleacetamide | Pantoea agglomerans | ? | - |
? | |
N-acetyl-L-aspartate + H2O | - |
Pantoea agglomerans | acetate + L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Pantoea agglomerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Pantoea agglomerans |
Organism | Comment | Expression |
---|---|---|
Pantoea agglomerans | expression is induced by growth on (indol-3-yl)acetyl-L-aspartate or N-acetyl-L-aspartate | up |