Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1.0 mM AgNO3, complete inactivation | Pseudomonas fluorescens | |
CdCl2 | 1.0 mM, complete inactivation | Pseudomonas fluorescens | |
HgCl2 | 1.0 mM, complete inactivation | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
hydroxyacetanilide | - |
Pseudomonas fluorescens | |
0.069 | - |
p-nitroacetanilide | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Pseudomonas fluorescens |
52500 | - |
1 * 52500, SDS-PAGE | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
ATCC 39004 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
319.2 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
4°C or 20°C, stable for 420 min | Pseudomonas fluorescens |
4°C, storage after freeze-drying in buffer containing 5% lactose, about 12% loss of activity | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxyacetanilide + H2O | - |
Pseudomonas fluorescens | hydroxyaniline + acetate | - |
? | |
p-nitroacetanilide + H2O | - |
Pseudomonas fluorescens | 4-nitroaniline + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52500, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas fluorescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 25°C: about 55% of maximal activity, 55°C: about 40% of maximal activity | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
20 min, complete inactivation | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | pH 7.0: about 50% of maximal activity, pH 12.0: about 30% of maximal activity | Pseudomonas fluorescens |