BRENDA - Enzyme Database show
show all sequences of 3.5.1.129

Biosynthesis of nucleoside diphosphoramidates in Campylobacter jejuni

Taylor, Z.; Brown, H.; Holden, H.; Raushel, F.; Biochemistry 56, 6079-6082 (2017)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni subsp. jejuni serotype O:2
Q0P8J7
-
-
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
Q0P8J7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
L-glutamine phosphate is a very poor substrate
749919
Campylobacter jejuni subsp. jejuni serotype O:2
?
-
-
-
-
additional information
L-glutamine phosphate is a very poor substrate
749919
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
?
-
-
-
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
749919
Campylobacter jejuni subsp. jejuni serotype O:2
cytidine 5'-diphosphoramidate + L-glutamate
-
-
-
?
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
749919
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
cytidine 5'-diphosphoramidate + L-glutamate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.55
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Campylobacter jejuni subsp. jejuni serotype O:2
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
L-glutamine phosphate is a very poor substrate
749919
Campylobacter jejuni subsp. jejuni serotype O:2
?
-
-
-
-
additional information
L-glutamine phosphate is a very poor substrate
749919
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
?
-
-
-
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
749919
Campylobacter jejuni subsp. jejuni serotype O:2
cytidine 5'-diphosphoramidate + L-glutamate
-
-
-
?
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
749919
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
cytidine 5'-diphosphoramidate + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.55
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Campylobacter jejuni subsp. jejuni serotype O:2
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
pH 8.0, 25C
Campylobacter jejuni subsp. jejuni serotype O:2
Other publictions for EC 3.5.1.129
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749919
Taylor
Biosynthesis of nucleoside di ...
Campylobacter jejuni subsp. jejuni serotype O:2, Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819
Biochemistry
56
6079-6082
2017
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