Application | Comment | Organism |
---|---|---|
synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expressed in enzyme deficient Bacillus megaterium strain UN-cat, 20fold higher expression rate than in host strain | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
57000 | - |
1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | Priestia megaterium | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
strain UN1 and enzyme deficient strain UN-cat | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Bacillus megaterium UN-cat | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
PAC | - |
Priestia megaterium |
penicillin G acylase | - |
Priestia megaterium |