Cloned (Comment) | Organism |
---|---|
gene ansB, expression in Escherichia coli strain BL21 (DE3), method evaluation, overview | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Yersinia pseudotuberculosis | - |
L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Yersinia pseudotuberculosis IP32953 | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pseudotuberculosis | Q66CJ2 | gene ansB | - |
Yersinia pseudotuberculosis IP32953 | Q66CJ2 | gene ansB | - |
Purification (Comment) | Organism |
---|---|
recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography | Yersinia pseudotuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h | Yersinia pseudotuberculosis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.7 | - |
pH 8.0, 37°C, purified enzyme | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Yersinia pseudotuberculosis | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Yersinia pseudotuberculosis IP32953 | L-aspartate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase II | - |
Yersinia pseudotuberculosis |
Ypa | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Yersinia pseudotuberculosis |
Organism | Comment | Expression |
---|---|---|
Yersinia pseudotuberculosis | optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h | up |