Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | stimulates | Acinetobacter calcoaceticus |
General Stability | Organism |
---|---|
freezing at -20°C, complete and irreversible loss of activity | Acinetobacter calcoaceticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Acinetobacter calcoaceticus | |
Na2SO4 | - |
Acinetobacter calcoaceticus | |
NaCl | - |
Acinetobacter calcoaceticus | |
NaN3 | - |
Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-Asn | - |
Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
4°C, pH 6.0, 0.1 M citrate buffer, stable for several weeks | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Asn + H2O | 30% of the activity with L-Asn | Acinetobacter calcoaceticus | D-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Acinetobacter calcoaceticus | L-Asp + NH3 | - |
? | |
L-Gln + H2O | to about the same extent as L-Asn | Acinetobacter calcoaceticus | L-Glu + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 h, little loss of activity | Acinetobacter calcoaceticus |