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Literature summary for 3.5.1.1 extracted from

  • Sakamoto, T.; Araki, C.; Beppu, T.; Arima, K.
    Partial purification and some properties of extracellular asparaginase from Candida utilis (1977), Agric. Biol. Chem., 41, 1359-1364.
No PubMed abstract available
Search for more literature for 3.5.1.1

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, 80% loss of activity. 3 mM, complete inhibition Cyberlindnera jadinii
pyruvate 10 mM, 26% inhibition Cyberlindnera jadinii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Cyberlindnera jadinii
-
-

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Asn + H2O
-
 Cyberlindnera jadinii D-Asp + NH3
-
 ?
L-Asn + H2O
-
 Cyberlindnera jadinii L-Asp + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 about Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 4.0-10.0, 20 h, stable Cyberlindnera jadinii
50
-
 pH 4.0-10.0, 10 min, stable Cyberlindnera jadinii
55
-
 pH 7.0, stable Cyberlindnera jadinii
60
-
 pH 5.0-7.0, 10 min, stable Cyberlindnera jadinii
65
-
 pH 7.0, 10 min, 50% loss of activity Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolysis of D-Asn and L-Asn Cyberlindnera jadinii

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of L-Asn. pH 4.0: about 40% of maximal activity, pH 8: about 50% of maximal activity, hydrolysis of D-Asn Cyberlindnera jadinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 50°C, 10 min, stable, 37°C, 20 h, stable Cyberlindnera jadinii
5 7 60°C, 10 min, stable Cyberlindnera jadinii