Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant E41Q in deficient mutant cell DELTAyme1, complementation only by the wild-type | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E541Q | site-directed mutagenesis, exchange in the active site, no complementation of deficient mutant cell DELTAyme1 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | strong | Saccharomyces cerevisiae | |
o-phenanthroline | strong | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | integral, a large domain is exposed to the intermembrane space | Saccharomyces cerevisiae | 5743 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Saccharomyces cerevisiae | proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
in complex with m-AAA protease Yta12p, EC 3.4.24.B18, complex is termed i-AAA protease | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | conserved metal-binding site HEXXH, selective degradation, mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa + H2O | in vitro import into the mitochondrion | Saccharomyces cerevisiae | 4 peptide fragments f1-f4 | product characterization | ? | |
protein + H2O | proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis | Saccharomyces cerevisiae | peptides | - |
? | |
residues 1-74 of subunit 2 of cytochrome oxidase + H2O | two-step procedure, in vitro import into the mitochondrion | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAA protease | - |
Saccharomyces cerevisiae |
M41.004 | Merops-ID | Saccharomyces cerevisiae |
YME1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Saccharomyces cerevisiae |