Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MON208 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method with 25% (v/v) polyethylene glycol 3350, 200 mM ammonium acetate, 100 mM Tris, pH 8.5 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-hydroxy-4-([4-[4-(trifluoromethyl)phenoxy]phenyl]sulfonyl)tetrahydro-2H-pyran-4-carboxamide | - |
Homo sapiens | |
TIMP-3 | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200 HR gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
in 50 mM HEPES, pH 8.0, 2 mM oxidized glutathione, 50 mM arginine, 10 mM CaCl2, 0.2 mM ZnCl2, and 1% protease inhibitors, at room temperature | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aggrecan + H2O | - |
Homo sapiens | ? | - |
? | |
K(6FAM)-DVQEFRGVTAVIRC(Qsy9)-KGK + H2O | - |
Homo sapiens | K-(6FAM)-DVQE + FRGVTAVIRC(Qsy9)-KGK | - |
? |
Synonyms | Comment | Organism |
---|---|---|
a disintegrin and metalloproteinase with thrombospondin motifs-5 | - |
Homo sapiens |
ADAMTS-5 | - |
Homo sapiens |
aggrecanase-2 | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000038 | - |
in 100 mM Tris-HCl, 100 mM NaCl, 0.01 mM CaCl2, 0.05% Brij, pH 7.5 | Homo sapiens | TIMP-3 | |
0.00029 | - |
in 100 mM Tris-HCl, 100 mM NaCl, 0.01 mM CaCl2, 0.05% Brij, pH 7.5 | Homo sapiens | N-hydroxy-4-([4-[4-(trifluoromethyl)phenoxy]phenyl]sulfonyl)tetrahydro-2H-pyran-4-carboxamide |