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Literature summary for 3.4.24.B10 extracted from
- ADAM 12, a disintegrin metalloprotease, interacts with insulin-like growth factor-binding protein-3 (2000), J. Biol. Chem., 275, 18574-18580.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the soluble enzyme in COS-1 cells and in 293-EBNA cell line, coexpression of soluble enzyme and insulin-like growth factor-binding protein-3 in a Saccharomyces cerevisiae two hybrid expression system | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| EDTA | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secreted soluble enzyme | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can substitute for Zn2+ | Homo sapiens | |
| Zn2+ | metalloprotease, dependent on, can be substituted by Ca2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, processed recombinant enzyme, SDS-PAGE, x * 96000, recombinant enzyme proform, SDS-PAGE | Homo sapiens |
| 96000 | - |
x * 68000, processed recombinant enzyme, SDS-PAGE, x * 96000, recombinant enzyme proform, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin-like growth factor binding protein-3 + H2O | Homo sapiens | in serum of pregnant women | ? | - |
? | |
| protein + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O43184 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, soluble recombinant protein from 293-EBNA cell and COS-1 cell medium | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | high expression level | Homo sapiens | - |
| serum | of pregnant women | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin-like growth factor binding protein-3 + H2O | - |
Homo sapiens | ? | - |
? | |
| insulin-like growth factor binding protein-3 + H2O | in serum of pregnant women | Homo sapiens | ? | - |
? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, processed recombinant enzyme, SDS-PAGE, x * 96000, recombinant enzyme proform, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| a disintegrin and metalloprotease-12 | - |
Homo sapiens |
| ADAM 12 | - |
Homo sapiens |
| M12.212 | Merops-ID | Homo sapiens |
| meltrin alpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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