Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 DE3 Star cells | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
SDS-PAGE | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q52NH3 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap HP nickel column chromatography, ammonium sulfate precipitation, and phenyl-Sepharose column chromatography | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
83 kDa full-length protective antigen + H2O | - |
Bacillus anthracis | 20 kDa N-terminal fragment of protective antigen + 63 kDa N-terminal fragment of protective antigen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lethal factor of anthrax toxin | the C-terminal region carries out the metalloprotease activity, while the N-terminal domain (residues 1-288) is responsible for protective antigen binding | Bacillus anthracis |