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Literature summary for 3.4.24.80 extracted from

  • Toth, M.; Osenkowski, P.; Hesek, D.; Brown, S.; Meroueh, S.; Sakr, W.; Mobashery, S.; Fridman, R.
    Cleavage at the stem region releases an active ectodomain of the membrane type 1 matrix metalloproteinase (2005), Biochem. J., 387, 497-506.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.80

Activating Compound

Activating Compound Comment Organism Structure
TAPI-1 leads to higher amounts of the MT1-MMP 32 kDa form in the media, also slightly increases the amount of the 50 kDa species of MT1-MMP Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in non-malignant monkey kidney epithelial BS-C-1 and CV-1 cells by co-infecting the cells with the vTF7-3 and vTF-MT1 vaccinia viruses Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information specific amino acid substitutions at the Val524-Ile525 site have no effect on shedding of the 50 kDa species, whereas deletion of the entire stem region deltastem-MT1, which lacks Pro509-Gly535, completely abrogates shedding of the 50 kDa species Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
BB94
-
 Homo sapiens
TAPI-1 inhibits the shedding of the MT1-MMP 18 kDa form Homo sapiens
TIMP-2 specificity of inhibitor binding of MT1-MMP, shedding of MT1-MMP ectodomain alters the balance of TIMP-2 between the cell surface and the pericellular space Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 immunoblot analysis of untreated cells, soluble MT1-MMP species, product of autocatalytic processing Homo sapiens
27000
-
 immunoblot analysis of cells regardless of TAPI-1 treatment, contains haemopexin-like domain, formed by autocatalytic processing of the 57 kDa active form Homo sapiens
32000
-
 immunoblot analysis of untreated cells Homo sapiens
50000
-
 immunoblot analysis of untreated cells, shed ectodomain Homo sapiens
53000
-
 immunoblot analysis, species of MT1-MMP isolated from a non-detergent extract of human breast carcinoma tissue, lack of the cytosolic tail Homo sapiens
57000
-
 immunoblot analysis of cells treated with TAPI-1, membrane anchored fragment shed in membrane vesicles Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
inhibitor-tethered affinity purification with BB94 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast carcinoma cell
-
 Homo sapiens
-
HT-1080 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galectin-3 cleaved to the 22 kDa degradation product when exposed to cells expressing membrane-anhored wild type MT1-MMP or the recombinant 50 kDa enzyme form Homo sapiens ?
-
 ?
pro-MMP-2 MT1-MMP accomplishes full pro-MMP-2 activation, cleavage within the prodomain at the Asn37-Leu38 peptide bond Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
membrane type 1 matrix metalloproteinase
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens