Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require plasminogen or any other bloodborne intermediate for activity | Agkistrodon contortrix contortrix |
Cloned (Comment) | Organism |
---|---|
chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells | Agkistrodon contortrix contortrix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains one zinc ion in the active center | Agkistrodon contortrix contortrix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Agkistrodon contortrix contortrix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | Agkistrodon contortrix contortrix | the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 | ? | - |
? | |
additional information | Agkistrodon contortrix contortrix | chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agkistrodon contortrix contortrix | P28891 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q-Sepharose column chromatography, gel filtration | Agkistrodon contortrix contortrix |
Renatured (Comment) | Organism |
---|---|
using 50 mM Tris-HCl, 0.5 M L-arginine, 1 M NaCl, 0.5 mM ZnCl2, 1 mM oxidized glutathione, 2 mM reduced glutathione, and 2 M urea at pH 7.0. After the protein is added, the pH is adjusted to 7.5, and the protein is left to refold at 4°C for approximately 24 h | Agkistrodon contortrix contortrix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Agkistrodon contortrix contortrix | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 | Agkistrodon contortrix contortrix | ? | - |
? | |
additional information | chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase | Agkistrodon contortrix contortrix | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Agkistrodon contortrix contortrix |