Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strains BL21(DE3) and M15 of GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, as well as pBoNTCS-Thro encoding full-length BoNT/C fused C-terminally to a Streptag and containing an Escherichia coli protease sensitive peptide between LC and HC | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
E1172A | the binding of radioactively-labeled, in vitro translated HCE E1172A to rat brain synaptosomes is highly decreased to 8.5% of wild-type levels, as well as the binding of Escherichia coli derived HCE mutants to isolated GT1b | Clostridium botulinum |
E1195A | the HCF mutant E1195A displays a diminished affinity of 31.6% to synaptosomes as well as a reduction of about 85% in binding to isolated GT1b compared to HCF wild-type | Clostridium botulinum |
W1224L | the binding of radioactively-labeled, in vitro translated HCE W1224L to rat brain synaptosomes is highly decreased to 3.7% of wild-type levels, as well as the binding of Escherichia coli derived HCE mutants to isolated GT1b | Clostridium botulinum |
W1250L | the HCF mutant W1250L displays a diminished affinity of 20.5% to synaptosomes as well as a reduction of about 85% in binding to isolated GT1b compared to HCF wild-type | Clostridium botulinum |
W1266L | the mutant HCA W1266L that lacks ganglioside binding, does not interfere with BoNT/A neurotoxicity | Clostridium botulinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the toxicity of the enzyme is reduced in absence of gangliosides | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium botulinum | BoNT serotypes bind to structure of ganglioside GT1b receptors, structure and binding specificities, modelling, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, and Strep-tagged pBoNTCS-Thro encoding from Escherichia coli by glutathione and streptavidin affinity chromatography, respectively | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | BoNT serotypes bind to structure of ganglioside GT1b receptors, structure and binding specificities, modelling, overview | Clostridium botulinum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the BoNT toxin is composed of a catalytic light chain, a heavy chain, and a translocation domain | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT/A | - |
Clostridium botulinum |
BoNT/B | - |
Clostridium botulinum |
BoNT/C | - |
Clostridium botulinum |
BoNT/D | - |
Clostridium botulinum |
BoNT/E | - |
Clostridium botulinum |
BoNT/F | - |
Clostridium botulinum |
BoNT/G | - |
Clostridium botulinum |
botulinum neurotoxin C | - |
Clostridium botulinum |
botulinum neurotoxin E | - |
Clostridium botulinum |
botulinum neurotoxin F | - |
Clostridium botulinum |
General Information | Comment | Organism |
---|---|---|
additional information | uptake of BoNT/A, B, E and G into cells requires a dual interaction with gangliosides and the synaptic vesicle proteins synaptotagmin or SV2. Interaction of SV2A, SV2B and SV2C with botulinum neurotoxin F HC, overview | Clostridium botulinum |