Protein Variants | Comment | Organism |
---|---|---|
A164V | mutation inconsequential to elastolysis | Homo sapiens |
A182G | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
D124Q | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
D124Q/A182G | combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens |
D124Q/I180S | combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens |
D124Q/M156E | combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant retains wild-type activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens |
D124Q/M156E/A182G | mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is highly decreased compared to wild-type, compared to triple mutant D124Q/M156E/F185Y and D124Q/M156E/T205K catalytic efficacy toward fEln-100 is highly decreased | Homo sapiens |
D124Q/M156E/F185Y | mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is not affected compared to wild-type, compared to triple mutant D124Q/M156E/I180S and D124Q/M156E/A182G catalytic efficacy toward fEln-100 is moderately decreased | Homo sapiens |
D124Q/M156E/I180S | mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is highly decreased compared to wild-type, compared to triple mutant D124Q/M156E/F185Y and D124Q/M156E/T205K catalytic efficacy toward fEln-100 is highly decreased | Homo sapiens |
D124Q/M156E/T205K | mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is not affected compared to wild-type, compared to triple mutant D124Q/M156E/I180S and D124Q/M156E/A182G catalytic efficacy toward fEln-100 is moderately decreased | Homo sapiens |
D200E | mutation inconsequential to elastolysis | Homo sapiens |
G166R | mutation inconsequential to elastolysis | Homo sapiens |
I180S | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
I255V | mutation inconsequential to elastolysis | Homo sapiens |
K148T | mutation inconsequential to elastolysis | Homo sapiens |
M103F | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased catalytic efficacy (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
M156E | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
M156E/A182G | kcat (fEln-100) is twice that of wild-type, mutant retains wild-type activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens |
M156E/I180S | combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens |
N153Y | mutation inconsequential to elastolysis | Homo sapiens |
R117S | mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased (kcat/Km) toward fEln-100 compared to wild-type | Homo sapiens |
S142E | mutation inconsequential to elastolysis | Homo sapiens |
V144A | mutation inconsequential to elastolysis | Homo sapiens |
V162S | mutation inconsequential to elastolysis | Homo sapiens |
Y132A | mutation inconsequential to elastolysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
fEln-100 | pH 7.5, 25°C, wild-type | Homo sapiens | |
0.00109 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
0.00114 | - |
fEln-100 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
0.00115 | - |
fEln-100 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
0.00115 | - |
fEln-100 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
0.00119 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
0.00127 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens | |
0.0013 | - |
fEln-100 | pH 7.5, 25°C, mutant I180S | Homo sapiens | |
0.00135 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
0.00145 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens | |
0.00171 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
0.00175 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
0.00239 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
0.0034 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
0.00399 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
0.00466 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
0.113 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens | |
0.113 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
0.118 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
0.119 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
0.124 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
0.125 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
0.125 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
0.128 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
0.129 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, wild-type | Homo sapiens | |
0.13 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
0.135 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
0.17 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant I180S | Homo sapiens | |
0.18 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
0.194 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
0.202 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
0.241 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fEln-100 + H2O | alpha-elastin | Homo sapiens | ? | - |
? | |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O | soluble Knight's peptide substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMP-12 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
0.001 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
0.0018 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
0.0024 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens | |
0.0037 | - |
fEln-100 | pH 7.5, 25°C, mutant I180S | Homo sapiens | |
0.0048 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
0.0052 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
0.0057 | - |
fEln-100 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
0.0065 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens | |
0.0072 | - |
fEln-100 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
0.0092 | - |
fEln-100 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
0.01 | - |
fEln-100 | pH 7.5, 25°C, wild-type | Homo sapiens | |
0.0117 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
0.0124 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
0.0143 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
0.021 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
6.8 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
8.4 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
9.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
10.6 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
13.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
15.6 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
16.2 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens | |
16.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
16.9 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
17.1 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
17.2 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
17.3 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, wild-type | Homo sapiens | |
18 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
18 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens | |
18.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
24.1 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant I180S | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
0.82 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
1.31 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
1.66 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
1.855 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens | |
2.86 | - |
fEln-100 | pH 7.5, 25°C, mutant I180S | Homo sapiens | |
3.57 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
4.21 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
4.5 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens | |
4.65 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
4.89 | - |
fEln-100 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
4.97 | - |
fEln-100 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
7.06 | - |
fEln-100 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
7.13 | - |
fEln-100 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
8.04 | - |
fEln-100 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
10.69 | - |
fEln-100 | pH 7.5, 25°C, wild-type | Homo sapiens | |
51.57 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/A182G | Homo sapiens | |
53.66 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/I180S | Homo sapiens | |
54.61 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/A182G | Homo sapiens | |
57.42 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/I180S | Homo sapiens | |
67.23 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/I180S | Homo sapiens | |
120.1 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant A182G | Homo sapiens | |
120.8 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant R117S | Homo sapiens | |
133.4 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/F185Y | Homo sapiens | |
133.8 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, wild-type | Homo sapiens | |
134.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E | Homo sapiens | |
135.2 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E | Homo sapiens | |
136.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q | Homo sapiens | |
141.8 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant I180S | Homo sapiens | |
145.4 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M156E/A182G | Homo sapiens | |
146.3 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant M103F | Homo sapiens | |
159.7 | - |
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 | pH 7.5, 25°C, mutant D124Q/M156E/T205K | Homo sapiens |