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Literature summary for 3.4.24.55 extracted from
- Pitrilysin (1995), Methods Enzymol., 248, 684-692.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | especially in the presence of zinc | Escherichia coli |  |
| Ca2+ | 1 mM, weak, reactivates after inhibition with chelating agents | Escherichia coli | |
| Co2+ | 1 mM | Escherichia coli | |
| dynorphin A(1-13) | - |
Escherichia coli | |
| Mn2+ | 1 mM, weak, reactivates after inhibition with chelating agents | Escherichia coli | |
| Zn2+ | 1 mM inhibits, but reactivates after inhibition with chelating agents | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
| Co2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
| Mn2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
| Zn2+ | most effective metal ion in reactivation after inhibition by chelating agent | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
1 * 116000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM Tris-HCl, pH 7.5, 40% v/v glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O | - |
Escherichia coli | ? | - |
? | |
| angiotensinogen(1-14) + H2O | - |
Escherichia coli | ? | - |
? | |
| Insulin B-chain + H2O | - |
Escherichia coli | ? | - |
? | |
| Secretin + H2O | - |
Escherichia coli | ? | - |
? | |
| Thyrocalcitonin + H2O | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 116000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
(7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
4-dinitrophenyl)Lys-Leu-Asp-D-Lys | Escherichia coli |
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Release 2023.1 (January 2023)
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