Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Benzyloxycarbonyl-Gly-Leu | - |
Protobothrops flavoviridis | |
Benzyloxycarbonyl-Gly-Leu-NH2 | - |
Protobothrops flavoviridis | |
Benzyloxycarbonyl-Gly-Pro-Leu | - |
Protobothrops flavoviridis | |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Protobothrops flavoviridis | |
Cd2+ | - |
Protobothrops flavoviridis | |
Cu2+ | - |
Protobothrops flavoviridis | |
cysteine | - |
Protobothrops flavoviridis | |
EDTA | - |
Protobothrops flavoviridis | |
Hg2+ | - |
Protobothrops flavoviridis | |
Ni2+ | - |
Protobothrops flavoviridis | |
PCMB | - |
Protobothrops flavoviridis | |
Zn2+ | - |
Protobothrops flavoviridis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro | - |
Protobothrops flavoviridis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | most active at ionic strength between 0.05 and 0.10 | Protobothrops flavoviridis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
Trimeresurus flavoviridis, low speed sedimentation | Protobothrops flavoviridis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Protobothrops flavoviridis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Protobothrops flavoviridis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Protobothrops flavoviridis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Protobothrops flavoviridis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O | - |
Protobothrops flavoviridis | Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro | - |
? | |
casein + H2O | - |
Protobothrops flavoviridis | hydrolyzed casein | - |
? | |
additional information | completely free from hemorrhagic acitivity | Protobothrops flavoviridis | ? | - |
? | |
Oxidized insulin B-chain + H2O | cleavage at Asn3-Gln4, His10-Leu11 and Ala14-Leu15 | Protobothrops flavoviridis | Hydrolyzed oxidized insulin B-chain | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Protobothrops flavoviridis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | about 30% of activity maximum at 30°C and 70°C | Protobothrops flavoviridis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 8.5, 5 min, enzyme retains full activity up to 45°C | Protobothrops flavoviridis |
60 | - |
pH 8.5, 5 min, complete loss of activity above | Protobothrops flavoviridis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
casein | Protobothrops flavoviridis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 6: about 40% of activity maximum, 11: about 80% of activity maximum | Protobothrops flavoviridis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
37°C, 1 h, about 40% loss of activity | Protobothrops flavoviridis |
7 | 9 | 37°C, 1 h, stable | Protobothrops flavoviridis |
11 | - |
37°C, 1 h, about 60% loss of activity | Protobothrops flavoviridis |