Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartate | - |
Chlamydomonas reinhardtii | |
CDTA | - |
Chlamydomonas reinhardtii | |
Co2+ | above 1-2 mM, native or apoenzyme | Chlamydomonas reinhardtii | |
Cu(CH3COO)2 | - |
Chlamydomonas reinhardtii | |
cysteine | - |
Chlamydomonas reinhardtii | |
EDTA | inactivation by dialysis, 1-2 mM Zn2+, Mn2+ or Co2+ restores activity of metall-free apoenzyme to 0.5%, 10% and 50% of native enzyme, respectively, no restoring by Mg2+, Ca2+, Cu2+ or Fe3+; most effectively reversed by Co2+ | Chlamydomonas reinhardtii | |
EGTA | about 25% as effective as EDTA or CDTA | Chlamydomonas reinhardtii | |
glutamate | - |
Chlamydomonas reinhardtii | |
histidine | - |
Chlamydomonas reinhardtii | |
Mn2+ | above 1-2 mM, native or apoenzyme | Chlamydomonas reinhardtii | |
additional information | chymostatin, antipain, leupeptin; pepstatin A; various amino acids, protease inhibitor E-64 | Chlamydomonas reinhardtii | |
phosphoramidon | - |
Chlamydomonas reinhardtii | |
Zn2+ | above 1-2 mM, native or apoenzyme | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not contain cobalt or manganese | Chlamydomonas reinhardtii | |
Zinc | zinc enzyme, 2.5 gatom zinc/62000 MW enzyme subunit, atomic absorption spectroscopy | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, Chlamydomonas reinhardtii, SDS-PAGE | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cell wall protein + H2O | Chlamydomonas reinhardtii | released during agglutination of gametes of opposite mating type | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
green alga, wild-type strain 137c | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from gametes | Chlamydomonas reinhardtii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | poor substrate | Chlamydomonas reinhardtii | fragments of azocasein | - |
? | |
casein + H2O | alpha- and beta-casein, poor substrate | Chlamydomonas reinhardtii | polypeptides derived from casein | - |
? | |
Cell wall protein + H2O | released during agglutination of gametes of opposite mating type | Chlamydomonas reinhardtii | ? | - |
? | |
Chlamydomonas cell wall polypeptide + H2O | 2 polypeptides (peptide 2, MW 294000, and 5, MW 215000) of 16 polypeptides, i.e. the NaClO4-insoluble fraction, disintegrates cell wall structure by acting on only a few salt-insoluble components | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | bovine serum albumin | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | no substrates are cytochrome c, lysozyme, myoglobin, trypsin inhibitor, ovalbumin | Chlamydomonas reinhardtii | ? | - |
? | |
RNAse A + H2O | - |
Chlamydomonas reinhardtii | Lower MW polypetides | - |
? | |
Zoosporangia cell walls + H2O | glutaraldehyde-fixed | Chlamydomonas reinhardtii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, Chlamydomonas reinhardtii, SDS-PAGE | Chlamydomonas reinhardtii |