Protein Variants | Comment | Organism |
---|---|---|
E198A | mutant enzyme is not activated by 4-aminophenylmercuric acetate, no collagen cleavage activity | Homo sapiens |
N188G | 25% decrease in collagen cleavage, 30% decrease in cleavage of (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-[3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-Ala-Arg-NH2 | Homo sapiens |
N190L | unstable | Homo sapiens |
Y189F | 88% of wild-type activity with (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-[3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-Ala-Arg-NH2. Activity against type I collagen dripps 3fold compared with wild-type enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, recombinant mutant enzymes are purified in the proenzyme form and all display an apparent molecular mass of 79000 Da, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | recombinant mutant enzymes are purified in the proenzyme form and all display an apparent molecular mass of 79000 Da, 4-aminophenylmercuric acetate-initiated autolytic processing to the fully activated form of the purified mutants, generation of the 59000 Da active enzyme after removal of the propeptide | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-[3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-Ala-Arg-NH2 + H2O | - |
Homo sapiens | ? | - |
? | |
Collagen + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, recombinant mutant enzymes are purified in the proenzyme form and all display an apparent molecular mass of 79000 Da, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MMP-8 | - |
Homo sapiens |