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Literature summary for 3.4.24.34 extracted from

  • Hasty, K.A.; Jeffrey, J.J.; Hibbs, M.S.; Welgus, H.G.
    The collagen substrate specificity of human neutrophil collagenase (1987), J. Biol. Chem., 262, 10048-10052.
    View publication on PubMed
Search for more literature for 3.4.24.34

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 guinea pig collagen type I 37°C Homo sapiens
0.0007
-
 human collagen type I 37°C Homo sapiens
0.0011
-
 human collagen type II 37°C Homo sapiens
0.0018
-
 human collagen type III 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
specific granule
-
 Homo sapiens 42581
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Homo sapiens initiates degradation of native collagen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant of phorbol myristate acetate stimulated neutrophils, immunoaffinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte neutrophil Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.13 mg fibrillar guinea pig collagen type I/mg enzyme/min, 0.09 mg human collagen type III/mg enzyme/min, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O initiates degradation of native collagen Homo sapiens ?
-
 ?
Fibrillar type I collagen + H2O from human or guinea pig Homo sapiens ?
-
 ?
Fibrillar type III collagen + H2O
-
 Homo sapiens ?
-
 ?
guinea pig collagen type I + H2O
-
 Homo sapiens ?
-
 ?
human collagen type I + H2O
-
 Homo sapiens ?
-
 ?
human collagen type II + H2O
-
 Homo sapiens ?
-
 ?
human collagen type III + H2O
-
 Homo sapiens ?
-
 ?
Monomeric type I collagen + H2O from human Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
Monomeric type I collagen + H2O from guinea pig Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
Monomeric type I collagen + H2O best substrate, unlike EC 3.4.24.7 this enzyme cleaves type III collagen more slowly than type I Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
Monomeric type I collagen + H2O catalyzes single cleavage in alpha-chain Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
Monomeric type II collagen + H2O
-
 Homo sapiens collagen type II fragment TCA + collagen type II fragment TCB three-quarter and one-quarter length fragments ?
Monomeric type III collagen + H2O unlike EC 3.4.24.7 this enzyme cleaves type III collagen more slowly than type I Homo sapiens collagen type III fragment TCA + collagen type III fragmentTCB three-quarter and one-quarter length fragments ?
additional information no hydrolysis of monomeric type IV or V collagen Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000233
-
 human collagen type III 37°C Homo sapiens
0.00065
-
 human collagen type II 37°C Homo sapiens
0.00161
-
 guinea pig collagen type I 37°C Homo sapiens
0.00178
-
 human collagen type I 37°C Homo sapiens