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Literature summary for 3.4.24.26 extracted from
- Isolation of a putative virulence agent, cytotoxic serine-elastase, from a newly isolated Pseudomonas aeruginosa ZuhP13 (2019), J. Biosci., 44, 7 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | slight inhibition | Pseudomonas aeruginosa |  |
| 2,2'-bipyridine | slight inhibition | Pseudomonas aeruginosa | |
| Aprotinin | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| Ba2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Ca2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Cd2+ | 5 mM, decreases activity | Pseudomonas aeruginosa | |
| Co2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Cu2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| EDTA | slight inhibition | Pseudomonas aeruginosa | |
| Elastatinal | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| Fe3+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Hg2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Mg2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| Mn2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| PMSF | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| Soybean trypsin inhibitor | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| TLCK | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| Zn2+ | slight inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
azocasein | kcat/Km: 1.3 mg/ml, pH 7.5, 40°C | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa ZuhP13 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| azocasein + H2O | - |
Pseudomonas aeruginosa ZuhP13 | ? | - |
? | |
| additional information | the enzyme shows both hemolytic and hemorrhagic activities in vivo | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | the enzyme shows both hemolytic and hemorrhagic activities in vivo | Pseudomonas aeruginosa ZuhP13 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 75% of maximal activity, 50°C: about 85% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life time: 297.7 min | Pseudomonas aeruginosa |
| 60 | - |
30 min, stable | Pseudomonas aeruginosa |
| 60 | - |
half-life time: 97.3 min | Pseudomonas aeruginosa |
| 61.3 | - |
midpoint temperature | Pseudomonas aeruginosa |
| 70 | - |
half-life time: 26.5 min | Pseudomonas aeruginosa |
| 80 | - |
half-life time: 0 min | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.27 | - |
azocasein | pH 7.5, 40°C | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 1 h, stable | Pseudomonas aeruginosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
5.6 |
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