Cloned (Comment) | Organism |
---|---|
gene MMP2, sequence comparisons, enzyme expression analysis | Homo sapiens |
gene MMP2, sequence comparisons, enzyme expression analysis | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Rattus norvegicus | |
Ca2+ | essential for gelatinase activity, no gelatinase activity at 1 mM Ca2+ | Homo sapiens | |
Zn2+ | zinc metalloprotease | Homo sapiens | |
Zn2+ | zinc metalloprotease | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
Gelatin + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08253 | - |
- |
Rattus norvegicus | P33436 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation of pro-MMP2 to MMP-2 | Rattus norvegicus |
proteolytic modification | p-aminophenylmercuric acetate (APMA) promotes activation of pro-MMP2 and its autocatalysis to active MMP2 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac muscle | - |
Rattus norvegicus | - |
commercial preparation | recombinant pro-enzyme and recombinant mature, activated enzyme | Homo sapiens | - |
additional information | in rat soleus muscle and other tissues, pro- and active MMP2 show gelatinase activity, as well as a higher band that has been reported previously to represent a rodent-specific glycosylated MMP2 | Rattus norvegicus | - |
skeletal muscle | distribution and activation of matrix metalloproteinase-2 in skeletal muscle fibers, quantification of MMP2 in muscle, overview | Homo sapiens | - |
skeletal muscle | distribution and activation of matrix metalloproteinase-2 in skeletal muscle fibers, quantification of MMP2 in muscle, overview. On average, about 57% of the MMP2, along with most of the GAPDH, in the skinned fibers is freely diffusible in the cytosol, about 6% of the MMP2 dissociates with the subsequent 10-min Triton X-100 treatment, and about 37% of the MMP2 remains in the skinned fiber | Rattus norvegicus | - |
soleus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gelatinase A | - |
Homo sapiens |
gelatinase A | - |
Rattus norvegicus |
Matrix metalloproteinase 2 | - |
Homo sapiens |
Matrix metalloproteinase 2 | - |
Rattus norvegicus |
matrix metalloproteinase-2 | - |
Homo sapiens |
matrix metalloproteinase-2 | - |
Rattus norvegicus |
MMP2 | - |
Homo sapiens |
MMP2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme exhibits gelatinolytic activity which is enhanced by p-aminophenylmercuric acetate (APMA). The APMA treated enzyme probably also produces proteolytic products of myosin and other muscle proteins | Homo sapiens |