Application | Comment | Organism |
---|---|---|
biotechnology | engineering of enzyme variants with targeted, high substrate specificity | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D208G | site-directed mutagenesis, the mutant enzyme shows increased specificity for the A-R cleavage site compared to the wild-type enzyme | Escherichia coli |
additional information | random mutagenesis of gene ompT, screening for mutant variants with altered cleavage specificity, e.g. mutant variants 1.2.19 and 1.3.19 exhibits higher specificity for the cleavage site A-R and lower specificity for R-R than the wild-type | Escherichia coli |
S223R | site-directed mutagenesis, the mutant enzyme shows increased specificity for the A-R cleavage site and overall reduced activity compared to the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant D208G | Escherichia coli | |
0.009 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant S223R | Escherichia coli | |
0.009 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant variant 1.2.19 | Escherichia coli | |
0.015 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant variant 1.3.19 | Escherichia coli | |
0.016 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant wild-type enzyme | Escherichia coli | |
0.055 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant wild-type enzyme | Escherichia coli | |
0.16 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant variant 1.3.19 | Escherichia coli | |
0.24 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant D208G | Escherichia coli | |
0.26 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant variant 1.2.19 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli | 19867 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
WCARVGKGRGR-NH2 + H2O | proteolytic cleavage of the peptide at the site A-R | Escherichia coli | WCA + RVGKGRGR-NH2 | - |
? | |
WEEGGRRIGRGGK-NH2 + H2O | proteolytic cleavage of the peptide at the site R-R, no activity of mutant S223R, preferred substrate of wild-type OmpT | Escherichia coli | WEEGGR + RIGRGGK-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ompT | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant variant 1.2.19 | Escherichia coli | |
0.031 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant wild-type enzyme | Escherichia coli | |
0.3 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant D208G | Escherichia coli | |
0.4 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant mutant variant 1.3.19 | Escherichia coli | |
1.6 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant D208G | Escherichia coli | |
1.7 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant variant 1.3.19 | Escherichia coli | |
2.2 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant variant 1.2.19 | Escherichia coli | |
2.3 | - |
WCARVGKGRGR-NH2 | 22°C, recombinant mutant S223R | Escherichia coli | |
8.8 | - |
WEEGGRRIGRGGK-NH2 | 22°C, recombinant wild-type enzyme | Escherichia coli |