Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the PMII zymogen utilizes a prosegment-catalyzed folding mechanism | Plasmodium falciparum |
Renatured (Comment) | Organism |
---|---|
differential scanning calorimetry reveals that the native state of PMII is irreversibly unfolded, and in the pH range of 6.5-8.0, PMII refolds to a denatured state with higher thermal stability than native state. The denatured state can also be formed upon partially unfolding PMII at pH 11.0 and 37°C for 2 h, followed by adjustment to a pH in the range of 6.5-8.0. While the denatured state can be folded/unfolded reversibly, the native state exists as a kinetically trapped state. The native state is kinetically trapped by an unfolding barrier of 25.5 kcal/mol, and yet once unfolded, is prevented from folding by a comparable folding barrier | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
EDANS-CO-CH2-CH2-CO-L-Ala-L-Leu-L-Glu-L-Arg-L-Met-L-Phe-L-Leu-L-Ser-L-Phe-L-Pro-Dap(DABCYL)-OH + H2O | - |
Plasmodium falciparum | ? | - |
? |