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Literature summary for 3.4.23.20 extracted from
- Penicillopepsin (1976), Methods Enzymol., 45, 434-452.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Leu-Gly-Leu | inhibits trypsinogen activation, activates cleavage of Leu-Tyr amide | Penicillium janthinellum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Penicillium janthinellum | |
| Benzyloxycarbonyl-Gln-Tyr | - |
Penicillium janthinellum | |
| Benzyloxycarbonyl-Glu | - |
Penicillium janthinellum | |
| Benzyloxycarbonyl-Val-Tyr | - |
Penicillium janthinellum | |
| Diazoacetyl DL-norleucine methyl ester | in presence of Cu2+ | Penicillium janthinellum | |
| K2PtCl6 | partial | Penicillium janthinellum | |
| Leu-Gly-Leu | inhibits trypsinogen activation, activates cleavage of Leu-Tyr amide | Penicillium janthinellum | |
| additional information | benzyloxycarbonyl-Phe; not: benzyloxycarbonyl-Tyr; p-bromophenacylbromide | Penicillium janthinellum | |
| pepstatin | - |
Penicillium janthinellum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0076 | - |
Trypsinogen | - |
Penicillium janthinellum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium janthinellum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
homologue of: chymosin | Penicillium janthinellum |
| additional information | - |
homologue of: pepsin (mammalian) | Penicillium janthinellum |
| 32000 | - |
Penicillium janthinellum | Penicillium janthinellum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium janthinellum | - |
NRRL 905, C.M.I. 75589 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium janthinellum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Penicillium janthinellum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| Room temperature, pH 4.9, several days | Penicillium janthinellum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| B-chain of S-sulfo-insulin + H2O | - |
Penicillium janthinellum | ? | - |
? | |
| Bovine serum albumin + H2O | cleaves 15% of the peptide bonds | Penicillium janthinellum | ? | - |
? | |
| bovine trypsinogen + H2O | - |
Penicillium janthinellum | ? | - |
? | |
| additional information | acts on some small substrates with two hydrophobic amino acids at the N-terminus and a free amino group, in addition to removing the N-terminal amino acid hydrolytically a trans-peptidation reaction occurs: Leu-Leu is formed from Leu-Tyr-Leu, Phe-Phe from Phe-Tyr-Thr-Pro-Lys-Ala and Met-Met from Met-Leu-Gly | Penicillium janthinellum | ? | - |
? | |
| Trypsinogen + H2O | bovine, activation by cleavage of Lys6-Ile7 | Penicillium janthinellum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 3.0 or pH 6.1, slow inactivation | Penicillium janthinellum |
| 55 | - |
pH 4.9, 1 h, stable | Penicillium janthinellum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
bovine trypsinogen | - |
Penicillium janthinellum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
bovine serum albumin | Penicillium janthinellum |
| 3.4 | - |
trypsinogen | Penicillium janthinellum |
| 3.6 | - |
hydrolysis and transpeptidation of small substrates | Penicillium janthinellum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | rapid loss of activity below pH 2.0 and above pH 7.0 | Penicillium janthinellum |
| 3 | - |
37°C, slow inactivation | Penicillium janthinellum |
| 4.9 | - |
55°C, 1 h, stable | Penicillium janthinellum |
| 6.1 | - |
37°C, slow inactivation | Penicillium janthinellum |
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