Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.1 extracted from

  • Leal, A.R.; Cruz, R.; Bur, D.; Huesgen, P.F.; Faro, R.; Manadas, B.; Wlodawer, A.; Faro, C.; Simoes, I.
    Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans (2016), Sci. Rep., 6, 23869 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Shewanella denitrificans

Protein Variants

Protein Variants Comment Organism
D37A inactive Shewanella denitrificans

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by Pefabloc, EDTA, E-64, amstatin, and dithiohtreitol Shewanella denitrificans
pepstatin complete inhibition at 0.001 mM Shewanella denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00867
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) at pH 4.0 and 37°C Shewanella denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Shewanella denitrificans 5737
-

Organism

Organism UniProt Comment Textmining
Shewanella denitrificans Q12R32
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+-carged His-Trap HP column chromatography, pepstatin-agarose affinity chromatography, and Mono Q column chromatography Shewanella denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) + H2O
-
 Shewanella denitrificans ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe + Phe-Ala-Leu-Lys-(2,4-dinitrophenyl)
-
 ?
additional information no activity with ((7-methoxycoumarin-4-yl)acetyl)-Lys-Leu-His-Pro-Glu-Val-Leu-Phe-Val-Leu-Glu-Lys-(2,4-dinitrophenyl), Arg-Glu-(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-(DABCYL)-Arg, ((7-methoxycoumarin-4-yl)acetyl)-Lys-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-(2,4-dinitrophenyl), or ((7-methoxycoumarin-4-yl)acetyl)-Lys-Ala-Leu-Ile-Pro-Ser-Tyr-Lys-Trp-Ser-Lys-(2,4-dinitrophenyl) Shewanella denitrificans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Shewanella denitrificans
? x * 49025, calculated from amino acid sequence Shewanella denitrificans

Synonyms

Synonyms Comment Organism
shewasin D
-
 Shewanella denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Shewanella denitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 maximum enzyme activity is detected between 30°C and 37°C. Above this temperature the activity abruptly decreases, with no detectable activity remaining at 50°C. At lower temperatures the activity decreases gradually, with the enzyme retaining 30% activity at 10°C Shewanella denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.51
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) at pH 4.0 and 37°C Shewanella denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4
-
 Shewanella denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
3 5 maximum activity towards this substrate at pH values between pH 3.5 and 4.0, with a drastic decrease in activity of 50% and 70% at pH values 3.0 and 5.0, respectively Shewanella denitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
400
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) at pH 4.0 and 37°C Shewanella denitrificans