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Literature summary for 3.4.23.1 extracted from

  • Miura, Y.; Kageyama, T.; Moriyama, A.
    Pepsinogens and pepsins from largemouth bass, Micropterus salmoides purification and characterization with special reference to high proteolytic activities of bass enzymes (2015), Comp. Biochem. Physiol. B, 183, 42-48 .
    View publication on PubMed
Search for more literature for 3.4.23.1

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
 Micropterus salmoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 hemoglobin isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides
0.0344
-
 Substance P isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides
0.039
-
 hemoglobin isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides
0.698
-
 Substance P isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides

Organism

Organism UniProt Comment Textmining
Micropterus salmoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sephacel column chromatography, Sephadex G-100 gel filtration, and Mono Q column chromatography Micropterus salmoides

Source Tissue

Source Tissue Comment Organism Textmining
gastric mucosa
-
 Micropterus salmoides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Micropterus salmoides ?
-
 ?
oxidized insulin B chain + H2O the cleavage specificities clarified with oxidized insulin B chain are restricted to a few bonds consisting of hydrophobic/aromatic residues, such as the Leu15-Tyr16, Phe24-Phe25 and Phe25-Tyr26 bonds Micropterus salmoides FVNQHLCGSHLVEAL + YLVCGERGFFYTPKA
-
 ?
Substance P + H2O RPKPQQFFGLM Micropterus salmoides ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, isoform PG1-1, SDS-PAGE Micropterus salmoides
? x * 41000, isoform PG2-2, SDS-PAGE Micropterus salmoides

Synonyms

Synonyms Comment Organism
PG1-1 isoform Micropterus salmoides
PG2-2 isoform Micropterus salmoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoform PG2-2 Micropterus salmoides
50
-
 isoform PG1-1 Micropterus salmoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.8
-
 Substance P isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides
52.7
-
 hemoglobin isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides
61
-
 Substance P isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides
62.9
-
 hemoglobin isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5
-
 isoform PG1-1, for hemoglobin digestion Micropterus salmoides
2
-
 isoform PG2-2, for hemoglobin digestion Micropterus salmoides

pH Range

pH Minimum pH Maximum Comment Organism
1.5 3.5 highly active between pH 1.5-3.5 Micropterus salmoides

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 at pH 2.0 and 14°C, isoform PG1-1 and PG2-2 are converted through intermediate forms into their respective pepsins. While the intermediate form of PG1-1 is rather stable, as the enzyme remained in that form for more than 60 min, the intermediate form of PG2-2 is converted to pepsin rapidly Micropterus salmoides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
91
-
 Substance P isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides
1140
-
 Substance P isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides
1600
-
 hemoglobin isoform PG1-1, at pH 3.0 and 37°C Micropterus salmoides
3500
-
 hemoglobin isoform PG2-2, at pH 3.0 and 37°C Micropterus salmoides