Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pepsinogen is converted into pepsin quickly at pH 2.0 | Monopterus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by E-64 and EDTA | Monopterus albus | |
pepstatin A | completely inhibited at a pepstatin/pepsin molar ratio of 1:2 | Monopterus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, active enzyme, SDS-PAGE | Monopterus albus |
36000 | - |
x * 36000, pepsinogen, SDS-PAGE | Monopterus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monopterus albus | - |
rice field eel | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacel gel filtration, and Sephacryl S-200 gel filtration | Monopterus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Monopterus albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the crude extract and 8.4fold purified enzyme are 1.8 and 15.1 units/mg. One unit of pepsin activity is defined as an increase of absorbance of 1.0 at 280 nm during an incubation time of 30 min at pH 3.0 and 37°C | Monopterus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Monopterus albus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, active enzyme, SDS-PAGE | Monopterus albus |
? | x * 36000, pepsinogen, SDS-PAGE | Monopterus albus |
Synonyms | Comment | Organism |
---|---|---|
pepsin 1 | isoform | Monopterus albus |
PG1 | pepsinogen isoform | Monopterus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the activity of pepsin isoform 1 is largely lost at higher than 50°C | Monopterus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.2 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Monopterus albus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Monopterus albus | pepsinogen 1, isoelectric focusing | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |