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Literature summary for 3.4.22.68 extracted from
- Immobilization of Ulp1 protease on NHS-activated Sepharose a useful tool for cleavage of the SUMO tag of recombinant proteins (2017), Biotechnol. Lett., 39, 1025-1031 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | immobilization of Ulp1 as a tool for cleavage of the SUMO tag of recombinant proteins. Ulp1 immobilized on N-hydroxysuccinimide-activated Sepharose maintains 95% substrate-cleavage ability and significantly enhanced pH and thermal stability. The immobilized Ulp1 can tolerate 15% (v/v) DMSO and 20% (v/v) ethanol. It can be reused for more than 15 batch reactions with 90% activity retention | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
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