Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Glu 54 | active role in proteolysis | Norwalk virus |
Application | Comment | Organism |
---|---|---|
medicine | Norwalk virus is the major cause of acute, epidemic, viral gastroenteritis | Norwalk virus |
Cloned (Comment) | Organism |
---|---|
cloned into pET41 Ek/LIC and pET46 Ek/LIC and expressed in Escherichia coli BL21 (DE3) | Norwalk virus |
Crystallization (Comment) | Organism |
---|---|
by hanging drop vapor diffusion or sitting drop vapor diffusion at room temperature, to 1.5 and 2.2 A resolution | Norwalk virus |
Protein Variants | Comment | Organism |
---|---|---|
E54A | Glu at the active centre | Norwalk virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)-benzene-sulfonyl fluoride | irreversible inhibition, soluble stable nontoxic alternative to phenylmethylsulfonyl fluoride | Norwalk virus | |
phenylmethylsulfonyl fluoride | - |
Norwalk virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Norwalk virus | Q83883 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity chromatography or size exclusion chromatography | Norwalk virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ORF1 polyprotein + H2O | - |
Norwalk virus | six nonstructural proteins for replication | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | size exclusion chromatography results | Norwalk virus |
Synonyms | Comment | Organism |
---|---|---|
NV protease | - |
Norwalk virus |
NVPro | - |
Norwalk virus |
viral cysteine protease | - |
Norwalk virus |