Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cordycepin | i.e. 3'-deoxyadenosine, cells treated with 100 mM and 1 mM for 24 h, expression of caspase-9, Western blot analysis | Mus musculus |
Application | Comment | Organism |
---|---|---|
pharmacology | studies on the mechanism of cordycepin-induced apoptosis in MA-10 Leydig tumor cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone | i.e. Z-VAD-FMK, treatment with 100 microM caspase inhibitor in combination with 100 microM cordycepin for 24 h suppresses sub-G1 phase in MA-10 cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | Mus musculus | mechanisms of cordycepin-induced apoptosis, activation of caspase-9, caspase-3 and caspase-7 | caspase-3 + ? | - |
? | |
pro-caspase-7 + H2O | Mus musculus | mechanisms of cordycepin-induced apoptosis, activation of caspase-9, caspase-3 and caspase-7 | caspase-7 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9R0T0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Leydig cell | - |
Mus musculus | - |
MA-10 cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Western blot analysis reveals cordycepin-induced expression of caspase-9, activation of caspase-3 and caspase-7, but not of caspase-8 in a time- and dose-dependent manner, cordycepin-induced apoptosis acts through a caspase-9 and caspase-3 and caspase-7 dependent pathway | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | mechanisms of cordycepin-induced apoptosis, activation of caspase-9, caspase-3 and caspase-7 | Mus musculus | caspase-3 + ? | - |
? | |
pro-caspase-7 + H2O | mechanisms of cordycepin-induced apoptosis, activation of caspase-9, caspase-3 and caspase-7 | Mus musculus | caspase-7 + ? | - |
? |