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Literature summary for 3.4.22.56 extracted from

  • McGonigal, K.; Tanha, J.; Palazov, E.; Li, S.; Gueorguieva-Owens, D.; Pandey, S.
    Isolation and functional characterization of single domain antibody modulators of caspase-3 and apoptosis (2008), Appl. Biochem. Biotechnol., 157, 226-236.
    View publication on PubMed
Search for more literature for 3.4.22.56

Activating Compound

Activating Compound Comment Organism Structure
VhhCasp32 increases the enzyme activity by 38% at 0.0047 mM, the VHH proteins, expresed in SHSY-5Y cells and isolated from heavy chain antibody variable domain, i.e. VHH phage display library, are specific to caspase 3 and antagonist and agonist of apoptosis, effects of transiently-expressed VhhCasp31 and VhhCasp32 intrabodies on oxidative-stress-induced apoptosis in SHSY-5Y cells, overview Homo sapiens

Application

Application Comment Organism
drug development the VHH proteins, isolated from heavy chain antibody variable domain, i.e. VHH phage display library, are antagonist and agonist of apoptosis and could have potential for the development of therapeutics for neurodegenerative diseases and cancer, respectively Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged caspase-3 in Escherichia coli, effects of transiently-expressed VhhCasp31 and VhhCasp32 intrabodies on oxidative-stress-induced apoptosis in SHSY-5Y cells, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
VhhCasp31 reduces the enzyme activity by 39% at 0.0047 mM and by 85.5% at 0.0145 mM, which is a concentration three times that of caspase 3, the VHH proteins, expresed in SHSY-5Y cells and isolated from heavy chain antibody variable domain, i.e. VHH phage display library, are specific to caspase 3 and antagonist and agonist of apoptosis, effects of transiently-expressed VhhCasp31 and VhhCasp32 intrabodies on oxidative-stress-induced apoptosis in SHSY-5Y cells, overview Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 x * 15000, recombinant, active processed enzyme, SDS-PAGE, x * 35000, recombinant, inactive unprocessed enzyme, SDS-PAGE Homo sapiens
35000
-
 x * 15000, recombinant, active processed enzyme, SDS-PAGE, x * 35000, recombinant, inactive unprocessed enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ICAD + H2O Homo sapiens activity of caspase-activated deoxyribonuclease, CAD, is affected by the caspase 3-mediated cleavage of the CAD inhibitor, ICAD ?
-
 ?
additional information Homo sapiens caspase 3 acts as executioner proteases causing proteolysis of key proteins leading to DNA fragmentation and apoptosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification caspases are synthesized in the cell as single-chain precursors and are activated by cleavage at the conserved Asp297 residue, where a conformational change occurs bringing it into the correct alignment for catalysis Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged caspase-3 from Escherichia coli by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DEVD-7-amido-4-fluoromethylcoumarin + H2O
-
 Homo sapiens DEVD + 7-amino-4-fluoromethylcoumarin
-
 ?
ICAD + H2O activity of caspase-activated deoxyribonuclease, CAD, is affected by the caspase 3-mediated cleavage of the CAD inhibitor, ICAD Homo sapiens ?
-
 ?
ICAD + H2O caspase 3-mediated cleavage of the caspase-activated deoxyribonuclease inhibitor, ICAD Homo sapiens ?
-
 ?
additional information caspase 3 acts as executioner proteases causing proteolysis of key proteins leading to DNA fragmentation and apoptosis Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 15000, recombinant, active processed enzyme, SDS-PAGE, x * 35000, recombinant, inactive unprocessed enzyme, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens