Any feedback?
Literature summary for 3.4.22.35 extracted from
- Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica (1999), Eur. J. Biochem., 266, 170-180.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | activates | Entamoeba histolytica |  |
| sulfhydryl compounds | activate | Entamoeba histolytica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-[(2S,3S)-carboxyoxiran-2-ylcarbonyl-L-leucylamido]butylguanidine | i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane or E64 | Entamoeba histolytica | |
| EDTA | slight inhibition at 10 mM | Entamoeba histolytica | |
| iodoacetamide | - |
Entamoeba histolytica | |
| additional information | no inhibition by pepstatin and phenylmethylsulfonyl fluoride | Entamoeba histolytica | |
| N-ethylmaleimide | - |
Entamoeba histolytica | |
| Phenanthroline | slight inhibition at 10 mM | Entamoeba histolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Entamoeba histolytica | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
native PAGE | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Entamoeba histolytica | - |
peptides | - |
? | |
| protein + H2O | Entamoeba histolytica HM1:IMSS | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
and its derivative clone A | - |
| Entamoeba histolytica HM1:IMSS | - |
and its derivative clone A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 15fold, to homogeneity | Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the enzyme is translocated to the amoebic surface upon the interaction of trophozoites with red blood cells, the enzyme participates thus in erythrophagocytosis | Entamoeba histolytica | - |
| trophozoite | - |
Entamoeba histolytica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2800 | - |
purified enzyme | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| Gelatin + H2O | - |
Entamoeba histolytica HM1:IMSS | ? | - |
? | |
| additional information | no activity with N-benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin and succinyl-LLVY-7-amido-4-methylcoumarin | Entamoeba histolytica | ? | - |
? | |
| additional information | no activity with N-benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin and succinyl-LLVY-7-amido-4-methylcoumarin | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
| N-benzyloxycarbonyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 17% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica | N-benzyloxycarbonyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| N-benzyloxycarbonyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 17% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica HM1:IMSS | N-benzyloxycarbonyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | N-benzyloxycarbonyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica HM1:IMSS | N-benzyloxycarbonyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| N-benzyloxycarbonyl-L-phenylalanyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 7% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| protein + H2O | - |
Entamoeba histolytica | peptides | - |
? | |
| protein + H2O | - |
Entamoeba histolytica HM1:IMSS | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000-60000, 3 forms, SDS-PAGE | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Entamoeba histolytica cysteine protease | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Entamoeba histolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | - |
Entamoeba histolytica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | isoelectric focusing | - |
6.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
