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Literature summary for 3.4.22.33 extracted from

  • Meza-Espinoza, L.; de Los Angeles Vivar-Vera, M.; de Lourdes Garcia-Magana, M.; Sayago-Ayerdi, S.G.; Chacon-Lopez, A.; Becerrea-Verdin, E.M.; Montalvo-Gonzalez, E.
    Enzyme activity and partial characterization of proteases obtained from Bromelia karatas fruit and compared with Bromelia pinguin proteases (2018), Food Sci. Biotechnol., 27, 509-517 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.33

Activating Compound

Activating Compound Comment Organism Structure
EDTA activates about 2.5fold Bromelia pinguin
additional information no effect on activity by EDTA Bromelia karatas

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Bromelia karatas
Hg2+
-
 Bromelia pinguin
additional information no effect on activity by EDTA Bromelia karatas
PMSF
-
 Bromelia karatas
PMSF
-
 Bromelia pinguin

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.235
-
 ovalbumin partially purified enzyme, pH 7.0, 37°C Bromelia pinguin
0.253
-
 ovalbumin partially purified enzyme, pH 7.0, 37°C Bromelia karatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000 31000 serine proteases Bromelia pinguin
21000 31000 serine proteases Bromelia karatas
66200 97000 cysteine proteases Bromelia pinguin
66200 97000 cysteine proteases Bromelia karatas

Organism

Organism UniProt Comment Textmining
Bromelia karatas
-
-
-
Bromelia pinguin
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 2.24fold Bromelia karatas
native enzyme 2.88fold Bromelia pinguin

Source Tissue

Source Tissue Comment Organism Textmining
fruit ripe fruits collected from wild species of Bromelia karatas in Cocuixitle, in Santiago Ixcuintla, Nayarit, Mexico Bromelia karatas
-
fruit ripe fruits collected from wild species of Bromelia pinguin in Guamara, in Santiago Ixcuintla, Nayarit, Mexico Bromelia pinguin
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.45
-
 crude enzyme extract, pH 7.0, 37°C, phosphate cysteine buffer Bromelia karatas
7.91
-
 crude enzyme extract, pH 7.0, 37°C, sodium sulfide buffer Bromelia pinguin
10
-
 partially purified enzyme, pH 7.0, 37°C, phosphate cysteine buffer Bromelia karatas
22.83
-
 partially purified enzyme, pH 7.0, 37°C, sodium sulfide buffer Bromelia pinguin

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
milk protein + H2O about 62% hydrolysis Bromelia karatas ?
-
 ?
milk protein + H2O about 69% hydrolysis Bromelia pinguin ?
-
 ?
ovalbumin + H2O about 50% hydrolysis Bromelia karatas ?
-
 ?
ovalbumin + H2O about 66% hydrolysis Bromelia pinguin ?
-
 ?
soybean protein + H2O about 73% hydrolysis Bromelia karatas ?
-
 ?
soybean protein + H2O about 82% hydrolysis Bromelia pinguin ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bromelia pinguin
37
-
 assay at Bromelia karatas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 partially purified enzyme, 120 min, pH 7.0, completely stable at Bromelia pinguin
37
-
 partially purified enzyme, 120 min, pH 7.0, completely stable at Bromelia karatas
40 50 partially purified enzyme, 120 min, pH 7.0, loss of 20% activity Bromelia pinguin
40 50 partially purified enzyme, 120 min, pH 7.0, loss of 20% activity Bromelia karatas
60
-
 partially purified enzyme, 120 min, pH 7.0, loss of 30% activity Bromelia pinguin
60
-
 partially purified enzyme, 120 min, pH 7.0, loss of 50% activity Bromelia karatas
70
-
 partially purified enzyme, 120 min, pH 7.0, loss of 70% activity Bromelia karatas
70
-
 partially purified enzyme, 120 min, pH 7.0, loss of 80% activity Bromelia pinguin

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bromelia pinguin
7
-
-
 Bromelia karatas

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 over 50% of maximal activity within this range Bromelia pinguin
5.5 8 over 50% of maximal activity within this range Bromelia karatas