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Literature summary for 3.4.22.28 extracted from

  • Yang, J.; Leen, E.N.; Maree, F.F.; Curry, S.
    Crystal structure of the 3C protease from Southern african territories type 2 foot-and-mouth disease virus (2016), PeerJ, 4, e1964 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
SAT1/NIG/5/81 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Foot-and-mouth disease virus SAT 1
SAT1/UGA/1/97 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Foot-and-mouth disease virus SAT 1
SAT2/GHA/8/91 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Foot-and-mouth disease virus SAT 2
SAT2/ZIM/7/83 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Foot-and-mouth disease virus SAT 2

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant detagged SAT1/N-g3Cpro(1-208), sitting drop vapour diffusion method, mixing of 100 nl of protein solution with 100 nl of reservoir solution, crystallization from mother liquor containing 15% w/v PEG 8000, 0.09 M Na-cacodylate pH 7.0, 0.27 M Ca-acetate, 0.01 M Tris, pH 8.5, and 0.08 M Na-thiocyanate, supplemented with 20% v/v glycerol, no crystals are obtained Foot-and-mouth disease virus SAT 1
recombinant detagged SAT2/G-g3Cpro(1-208), sitting drop vapour diffusion method, mixing of 100 nl of protein solution with 100 nl of reservoir solution, crystallization from mother liquor containing 15% w/v PEG 8000, 0.09 M Na-cacodylate pH 7.0, 0.27 M Ca-acetate, 0.01 M Tris, pH 8.5, and 0.08 M Na-thiocyanate, supplemented with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 3.2 A resolution Foot-and-mouth disease virus SAT 2
recombinant detagged SAT2/Zg3Cpro(1-208), sitting drop vapour diffusion method, mixing of 100 nl of protein solution with 100 nl of reservoir solution, crystallization from mother liquor containing 15% w/v PEG-8000, 0.09 M Na-cacodylate pH 7.0, 0.27 M Ca-acetate, 0.01 M Tris, pH 8.5, and 0.08 M Na-thiocyanate, supplemented with 20% v/v glycerol, no crystals are obtained Foot-and-mouth disease virus SAT 2

Protein Variants

Protein Variants Comment Organism
K110T/K111Y site-directed mutagenesis, the mutant produces significantly larger void peaks during purification by gel filtration chromatography than the wild-type Foot-and-mouth disease virus SAT 2
K110Y/K111T site-directed mutagenesis, the mutant appears almost entirely aggregated under the conditions used, no useable crystals are obtained Foot-and-mouth disease virus SAT 2
K51A/K54Y site-directed mutagenesis, the K51A/K54Y mutant gives protein that is as soluble as wild-type Foot-and-mouth disease virus SAT 2
K51T/K54S site-directed mutagenesis, the mutant produces significantly larger void peaks during purification by gel filtration chromatography than the wild-type Foot-and-mouth disease virus SAT 2
yes recombinant detagged SAT1/U-g3Cpro(1-208), sitting drop vapour diffusion method, mixing of 100 nl of protein solution with 100 nl of reservoir solution, crystallization from mother liquor containing 15% w/v PEG 8000, 0.09 M Na-cacodylate pH 7.0, 0.27 M Ca-acetate, 0.01 M Tris, pH 8.5, and 0.08 M Na thiocyanate, supplemented with 20% v/v glycerol, no crystals are obtained Foot-and-mouth disease virus SAT 1

Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus SAT 1 Q1EFA8 FMDV SAT1/NIG/5/81, a Southern African territories type 1 serotype
-
Foot-and-mouth disease virus SAT 1 Q91CA5 FMDV SAT1/UGA/1/97, a Southern African territories type 1 serotype
-
Foot-and-mouth disease virus SAT 2 Q1EF85 FMDV SAT2/GHA/8/91, a Southern African territories type 2 serotype
-
Foot-and-mouth disease virus SAT 2 Q719N0 FMDV SAT2/ZIM/7/83, a Southern African territories type 2 serotype
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS lysed cells by protamin treatment, metal affinity chromatography, thrombin treatment for tag cleavage, and dialysis followed by gel filtration Foot-and-mouth disease virus SAT 2
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS lysed cells by protamin treatment, metal affinity chromatography, thrombin treatment for tag cleavage, and dialysis followed by gel filtration Foot-and-mouth disease virus SAT 1

Synonyms

Synonyms Comment Organism
3C protease
-
 Foot-and-mouth disease virus SAT 2
3C protease
-
 Foot-and-mouth disease virus SAT 1
3Cpro
-
 Foot-and-mouth disease virus SAT 2
3Cpro
-
 Foot-and-mouth disease virus SAT 1
SAT-type 3C protease
-
 Foot-and-mouth disease virus SAT 2

General Information

General Information Comment Organism
evolution viruses belonging to the SAT serotypes display appreciably greater genomic and antigenic variation in their capsid proteins compared to Asia1 serotype A and O viruses, possibly due to their long term maintenance within African buffalo (Syncerus caffer). In common with other members of the picornavirus family, FMDV has a singlestranded, positive-sense RNA genome Foot-and-mouth disease virus SAT 2
evolution viruses belonging to the SAT serotypes display appreciably greater genomic and antigenic variation in their capsid proteins compared to Asia1 serotype A and O viruses, possibly due to their long term maintenance within African buffalo (Syncerus caffer). In common with other members of the picornavirus family, FMDV has a singlestranded, positive-sense RNA genome Foot-and-mouth disease virus SAT 1