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Literature summary for 3.4.22.2 extracted from

  • Zhang, B.; Li, P.; Zhang, H.; Fan, L.; Wang, H.; Li, X.; Tian, L.; Ali, N.; Ali, Z.; Zhang, Q.
    Papain/Zn3(PO4)2 hybrid nanoflower Preparation, characterization and its enhanced catalytic activity as an immobilized enzyme (2016), RSC Adv., 6, 46702-46710 .
No PubMed abstract available
Search for more literature for 3.4.22.2

Application

Application Comment Organism
industry catalytic properties of papain immobilized on hybrid nanoflowers are enhanced compared with that of free papain. The hybrid nanoflowers exhibit excellent reusability, high thermostability, long storage life and great potential in industrial applications Carica papaya

General Stability

General Stability Organism
papain immobilized on hybrid nanoflowers retains 88.8% of its initial activity after ten successive cycles of reuse Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 casein 0.245 g/ml (soluble enzyme), 0.252 g/ml (papain immobilized on hybrid nanoflowers), pH 7.4, 37°C Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya P00784
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Carica papaya ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 soluble enzyme Carica papaya
70
-
 papain immobilized on hybrid nanoflowers Carica papaya

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 25°C: about 65% of maximal activity, 70°C: about 60% of maximal activity, soluble enzyme Carica papaya
25 90 25°C: about 50% of maximal activity, 90°C: about 70% of maximal activity, papain immobilized on hybrid nanoflowers Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 390 min, the soluble enzyme retains 41.9% of its activity, enzyme immobilized on hybrid nanoflowers retains 77.2% of its activity Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 soluble enzyme Carica papaya
7.2
-
 papain immobilized on hybrid nanoflowers Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
6.4 9.4 pH 6.4: about 85% of maximal activity, pH 9.4: about 50% of maximal activity Carica papaya
6.4 10.2 pH 6.4: about 85% of maximal activity, pH 10.2: about 70% of maximal activity Carica papaya