Cloned (Comment) | Organism |
---|---|
expression of wild-type and catalytically inactive mutant full-length recombinant human MASP-1 entirely in the zymogen form in Hep-G2 and Chang liver cells or HEK-293F cells. The active protease is produced through co-expression with the serine protease inhibitor C1 inhibitor avoiding the self-suppression of enzyme expression by its autocatalytic activity, the expressed protease is capable of binding MBL and autoactivating, and is catalytically active | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S646A | site-directed mutagenesis, the mutant remains as inactive zymogen | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C1 inhibitor | C1INH, a serine protease inhibitor | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, enzyme-bound | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proform mannan-binding lectin-associated serine protease 1 + H2O | Homo sapiens | autocatalytic cleavage, a complement substrate, activation | mature mannan-binding lectin-associated serine protease 1 + ? | - |
? | |
proform mannan-binding lectin-associated serine protease 2 + H2O | Homo sapiens | a complement substrate, activation | mature mannan-binding lectin-associated serine protease 2 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48740 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | enzyme MASP-1 performs autoactivation through proteolytic cleavage of its zymogen. Natural MASP-1 is activated by cleavage in the activation peptide, generating an A-chain (CUB-EGF-CUB-CCPCCP) and a B-chain (the serine protease domain), which remain associated through a disulphide bridge. MASP-1 autodegradation occurs intracellularly, not inhibited by addition of inhibitors to the culture medium | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
proform mannan-binding lectin-associated serine protease 1 + H2O | autocatalytic cleavage, a complement substrate, activation | Homo sapiens | mature mannan-binding lectin-associated serine protease 1 + ? | - |
? | |
proform mannan-binding lectin-associated serine protease 2 + H2O | a complement substrate, activation | Homo sapiens | mature mannan-binding lectin-associated serine protease 2 + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme monomer domains are CUB1-EGF-CUB2-CCP1-CCP2-SP, standing for C1r/C1s-Uegf-BMP domain 1, epidermal growth factor domain, C1r/C1s-Uegf-BMP domain 2, complement control protein domain 1, complement control protein domain 2, and serine protease domain, domain organization of MASP-1, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MASP-1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | the catalytic activity of MASP-1 suppresses its expression through rapid auto-activation and autodegradation intracellularly, not inhibited by addition of inhibitors to the culture medium | down |
General Information | Comment | Organism |
---|---|---|
metabolism | MASP-1 is a protease of the lectin pathway of complement | Homo sapiens |
additional information | the catalytic activity of MASP-1 suppresses its expression through intracellular rapid autoactivation and autodegradation | Homo sapiens |