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Literature summary for 3.4.21.B1 extracted from

  • Hunfeld, A.; Etscheid, M.; Konig, H.; Seitz, R.; Dodt, J.
    Detection of a novel plasma serine protease during purification of vitamin K-dependent coagulation factors (1999), FEBS Lett., 456, 290-294.
    View publication on PubMed
Search for more literature for 3.4.21.B1

Inhibitors

Inhibitors Comment Organism Structure
alpha1-proteinase less efficient inhibition Homo sapiens
alpha2-antiplasmin most efficient inhibition Homo sapiens
Aprotinin most efficient inhibition Homo sapiens
C1-esterase inhibitor less efficient inhibition Homo sapiens
Co2+ inhibits at 2 mM Homo sapiens
Cu2+ inhibits at 2 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 DL-pyroglutamic acid-cyclohexylglycine-Arg 4-nitroanilide pH 8.2, 37°C Homo sapiens
0.58
-
 D-Phe-Pip-Arg-4-nitroanilide pH 8.2, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strong dependence, 3.5-4fold increase of activity at 1 mM Homo sapiens
Mg2+ activates at 2 mM Homo sapiens
Mn2+ activates at 2 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 50000 + 1 * 30000, SDS-PAGE Homo sapiens
50000
-
 1 * 50000 + 1 * 30000, SDS-PAGE Homo sapiens
70000
-
 PAGE under non-denaturating conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q14520
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg-4-nitroanilide + H2O i.e. A-2222, weak chromogenic substrate Homo sapiens benzoyl-Ile-Glu(gamma-OR)-Gly-Arg + 4-nitroaniline
-
 ?
CH2SO2-D-cyclohexylalanine-butanoyl-Arg-4-nitroanilide + H2O i.e. Pefa-5979, chromogenic substrate Homo sapiens CH2SO2-D-cyclohexylalanine-butanoyl-Arg + 4-nitroaniline
-
 ?
CH2SO2-D-cyclohexylglycine-Gly-Arg-4-nitroanilide i.e. Pefa-3107, chromogenic substrate Homo sapiens CH2SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline
-
 ?
CH3-OCO-D-cyclohexylalanine-Gly-Arg-4-nitroanilide + H2O i.e. Pefa-5523, chromogenic substrate Homo sapiens CH3-OCO-D-cyclohexylalanine-Gly-Arg + 4-nitroaniline
-
 ?
D-Ala-cyclohexyltyrosine-Lys-4-nitroanilide + H2O i.e. Pefa-5329, weak chromogenic substrate Homo sapiens D-Ala-cyclohexyltyrosine-Lys + 4-nitroaniline
-
 ?
D-cyclohexylglycine-Ala-Arg-4-nitroanilide + H2O i.e. Pefa-5114, good chromogenic substrate Homo sapiens D-cyclohexylglycine-Ala-Arg + 4-nitroaniline
-
 ?
D-Lys(benzyloxycarbonyl)-Pro-Arg-4-nitroanilide + H2O i.e. Pefa-5773, chromogenic substrate Homo sapiens D-Lys(benzyloxycarbonyl)-Pro-Arg + 4-nitroaniline
-
 ?
D-Phe-Pip-Arg-4-nitroanilide + H2O i.e. S-2238, chromogenic substrate Homo sapiens D-Phe-Pip-Arg + 4-nitroaniline
-
 ?
DL-pyroglutamic acid-cyclohexylglycine-Arg-4-nitroanilide + H2O i.e. Pefa-3297, good chromogenic substrate Homo sapiens DL-pyroglutamic acid-cyclohexylglycine-Arg + 4-nitroaniline
-
 ?
L-pyroglutamic acid-Pro-Arg-4-nitroanilide + H2O i.e. S-2366, chromogenic substrate Homo sapiens L-pyroglutamic acid-Pro-Arg + 4-nitroaniline
-
 ?
Protein + H2O
-
 Homo sapiens ?
-
 ?
Z-D-Arg-Gly-Arg-4-nitroanilide + H2O i.e. S-2765, good chromogenic substrate Homo sapiens Z-D-Arg-Gly-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 50000 + 1 * 30000, SDS-PAGE Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000088
-
 Aprotinin pH 8.2, 37°C Homo sapiens