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Literature summary for 3.4.21.98 extracted from

  • Nall, T.A.; Chappell, K.J.; Stoermer, M.J.; Fang, N.X.; Tyndall, J.D.; Young, P.R.; Fairlie, D.P.
    Enzymatic characterization and homology model of a catalytically active recombinant West Nile virus NS3 protease (2004), J. Biol. Chem., 279, 48535-48542.
    View publication on PubMed
Search for more literature for 3.4.21.98

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of enzyme based on structures of dengue-2 and hepatitis C viral proteases West Nile virus

Inhibitors

Inhibitors Comment Organism Structure
Ac-FASGKR-arginylaldehyde competitive, 50% inhibition at about 0.001 mM West Nile virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 Ac-FASGKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
0.74
-
 Ac-DPNRKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
0.821
-
 Ac-KPGLKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
0.892
-
 Ac-LQYTKR-4-nitroanilide 37°C, pH 9.5 West Nile virus

Organism

Organism UniProt Comment Textmining
West Nile virus
-
 recombinant protein consisting of a 40-residue component of cofactor NS2B tethered via a noncleavable nonapeptide to the N-terminal 184 residues of enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DPNRKR-4-nitroanilide + H2O
-
 West Nile virus ?
-
 ?
Ac-FASGKR-4-nitroanilide + H2O
-
 West Nile virus ?
-
 ?
Ac-KPGLKR-4-nitroanilide + H2O
-
 West Nile virus ?
-
 ?
Ac-LQYTKR-4-nitroanilide + H2O
-
 West Nile virus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 Ac-LQYTKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
1.3
-
 Ac-DPNRKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
1.5
-
 Ac-KPGLKR-4-nitroanilide 37°C, pH 9.5 West Nile virus
1.9
-
 Ac-FASGKR-4-nitroanilide 37°C, pH 9.5 West Nile virus