Protein Variants | Comment | Organism |
---|---|---|
K136M | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 23% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 80% of the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 7.8fold higher than the wild-type value | Hepacivirus C |
K136M/R109Q | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 12% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 7.8fold higher than the wild-type value | Hepacivirus C |
K165S | the turnover number for EAGDDIVPCSMSYTWTGA-OH is nearly identical to the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 1.53fold higher than the wild-type value | Hepacivirus C |
K165S/R161S | the turnover number for EAGDDIVPCSMSYTWTGA-OH is nearly identical to the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 10.8fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 4.5fold higher than the wild-type value | Hepacivirus C |
R109Q | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 60% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 3.64fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.9fold higher than the wild-type value | Hepacivirus C |
R123T | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 1.2fold higher than the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.5fold higher than the wild-type value | Hepacivirus C |
R155S | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 78% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 3.2fold higher than the wild-type value | Hepacivirus C |
R161S | the turnover number for EAGDDIVPCSMSYTWTGA-OH is 1.1fold higher than the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.4fold higher than the wild-type value | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | - |
Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K136M | Hepacivirus C | |
0.0005 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K165S | Hepacivirus C | |
0.0005 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.001 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R161S | Hepacivirus C | |
0.0012 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K136M/R109Q | Hepacivirus C | |
0.0012 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R123T | Hepacivirus C | |
0.0012 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R155S | Hepacivirus C | |
0.0018 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R109Q | Hepacivirus C | |
0.0038 | - |
EAGNDIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.0041 | - |
EAGDNIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.0054 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K165S/R161S | Hepacivirus C | |
0.0252 | - |
EAGNNIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hepatitis C virus polyprotein + H2O | Hepacivirus C | mutational cleavage | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
EAGDDIVPCSMSYTWTGA-OH + H2O | - |
Hepacivirus C | EAGDDIVPC + SMSYTWTGA-OH | - |
? | |
EAGDNIVPCSMSYTWTGA-OH + H2O | - |
Hepacivirus C | EAGDNIVPC + SMSYTWTGA-OH | - |
? | |
EAGNDIVPCSMSYTWTGA-OH + H2O | - |
Hepacivirus C | EAGNDIVPC + SMSYTWTGA-OH | - |
? | |
EAGNNIVPCSMSYTWTGA-OH + H2O | - |
Hepacivirus C | EAGNNIVPC + SMSYTWTGA-OH | - |
? | |
hepatitis C virus polyprotein + H2O | mutational cleavage | Hepacivirus C | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K136M/R109Q | Hepacivirus C | |
0.167 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K136M | Hepacivirus C | |
0.59 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R109Q | Hepacivirus C | |
0.67 | - |
EAGNNIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.7 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.7 | - |
EAGNDIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.72 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K165S/R161S | Hepacivirus C | |
0.77 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R161S | Hepacivirus C | |
0.78 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R155S | Hepacivirus C | |
0.8 | - |
EAGDNIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C | |
0.85 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R123T | Hepacivirus C | |
0.95 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K165S | Hepacivirus C | |
6.08 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme K165S/R161S | Hepacivirus C | |
6.08 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R109Q | Hepacivirus C | |
6.08 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R155S | Hepacivirus C | |
6.08 | - |
EAGDDIVPCSMSYTWTGA-OH | pH 7.5, mutant enzyme R161S | Hepacivirus C | |
6.08 | - |
EAGNNIVPCSMSYTWTGA-OH | pH 7.5, wild-type enzyme | Hepacivirus C |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000055 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme R161S | Hepacivirus C | |
0.00006 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme R123T | Hepacivirus C | |
0.0000615 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme K165S | Hepacivirus C | |
0.000079 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme R109Q | Hepacivirus C | |
0.000129 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme R155S | Hepacivirus C | |
0.000179 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme K165S/R1161S | Hepacivirus C | |
0.00031 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme K136M/R109Q | Hepacivirus C | |
0.000313 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, mutant enzyme K136M | Hepacivirus C | |
0.04 | - |
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH | pH 7.5, wild-type enzyme | Hepacivirus C |