Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dodecylphosphocholine | at 37°C, degradation occurs to a lesser extent in dodecylphosphocholine micelles than in Triton X-100 or n-octyl-beta-D-glucopyranoside | Escherichia coli | |
additional information | no significant difference in expression levels in the presence and absence of deuterium oxide. Elevated temperature enhances enzyme degradation, at 37°C, the protein is autolysed and degraded more than at 22°C | Escherichia coli | |
n-octyl-beta-D-glucopyranoside | - |
Escherichia coli | |
Triton X-100 | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells transfected with the pET23b-coding SPase I plasmid | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane domain | Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, gel filtration and on Ni-agarose resin | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cleaves the signal peptide of the GST-SP-AP-His construct into two fragments, the GST protein plus the signal peptide (28 kDa), and the first 30 amino acids of the mature region 6-His-tagged (4 kDa) | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Spase I | - |
Escherichia coli |
type I signal peptidase | - |
Escherichia coli |