Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | inactive proPSA enzyme can be activated by recombinant kallikrein 2 to the mature form | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | the size of the fraction of free enzyme in serum is a marker to distinguish benign hyperplasia from malignant carcinoma | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
serum | inhibits the mature enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28110 | 30000 | inactive zymogen peak 2, gel filtration, SDS-PAGE and mass spectrometry | Homo sapiens |
28880 | 30000 | active mature enzyme, gel filtration and mass spectrometry | Homo sapiens |
87000 | - |
inactive zymogen peak 1, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme can be activated by recombinant kallikrein 2 | ? | - |
? | |
polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
2 isozyme of the zymogen with different pI, several isoforms with different pIs of the mature enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | enzyme can be activated by recombinant kallikrein 2 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
from LNCaP cell culture medium | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate | - |
Homo sapiens | - |
prostate cancer cell | very low content of free enzyme | Homo sapiens | - |
prostate cancer cell | produces equal amounts of zymogen proPSA and one-chain mature enzyme form, no intermediate forms | Homo sapiens | - |
prostate cancer cell | metastatic prostate adenocarcinoma cell line LNCaP | Homo sapiens | - |
serum | enzyme is mainly complexed to alpha1-antichymotrypsin, i.e. ACT, a small part of free enzyme | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KGISSQY-7-amido-4-methylcoumarin + H2O | fluorogenic substrate | Homo sapiens | KGISSQY + 7-amino-4-methylcoumarin | - |
? | |
additional information | enzyme can be activated by recombinant kallikrein 2 | Homo sapiens | ? | - |
? | |
PFR-7-amido-4-methylcoumarin + H2O | fluorogenic substrate | Homo sapiens | PFR + 7-amino-4-methylcoumarin | - |
? | |
polypeptide + H2O | - |
Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | active mature enzyme consists of only 1 chain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
K3 | - |
Homo sapiens |
prostate specific antigen | - |
Homo sapiens |
prostate-specific antigen | - |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | several isozymes of active mature enzyme | - |
6.4 |
Homo sapiens | 2 enzyme proforms with different pI: pH 8.2 and pH 8.4 | - |
8.2 |
Homo sapiens | 2 enzyme proforms with different pI: pH 8.2 and pH 8.4 | - |
8.4 |