Cloned (Comment) | Organism |
---|---|
furin cDNA ligated into a modified pCEP-Pu vector carrying a 5'-SPARC/BM40 signal peptide and a 3'-FLAG tag. HEK 293-EBNA cells cotransfected with full-length alpha1(XXIII) or furin cDNA and phosphatidylinositol-linked placental alkaline phosphatase or human transferrin receptor cDNA, respectively | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AEBSF | partially inhibits ectodomain shedding by 45% | Mus musculus | |
alpha1-antitrypsin | - |
Mus musculus | |
antipain | minimal inhibition of ectodomain shedding | Mus musculus | |
chymostatin | minimal inhibition of ectodomain shedding | Mus musculus | |
decanoyl-Arg-Val-Lys-Arg-chloromethyl ketone | efficiently inhibits ectodomain shedding by 95% | Mus musculus | |
E-64 | minimal inhibition of ectodomain shedding | Mus musculus | |
leupeptin | minimal inhibition of ectodomain shedding | Mus musculus | |
additional information | pepstatin A, 1,10-ortho-phenanthroline, EDTA, or TAPI-0 do not inhibit ectodomain shedding | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Mus musculus | 5794 | - |
additional information | localization of furin predominantly outside lipid rafts | Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
membrane-bound collagen XXIII + H2O | furin is the major protease to process collagen XXIII. Processing occurs after the downstream recognition motif 94KIRTVR99, releasing the ectodomain | Mus musculus | shed collagen XXIII | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Proprotein convertase | - |
Mus musculus |
sheddase | - |
Mus musculus |