Literature summary for 3.4.21.64 extracted from

Stone, L.A.; Jackson, G.S.; Collinge, J.; Wadsworth, J.D.; Clarke, A.R.
Inhibition of proteinase K activity by copper(II) ions (2007), Biochemistry, 46, 245-252.

Search for more literature for 3.4.21.64

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	presence of calcium ions, up to 10 mM, has no effect on enzymatic activity	Tritirachium album Limber

Application

Application	Comment	Organism
medicine	PK activity in the presence of brain correlates precisely with the concentration of Cu2+ ions that prevent PK digestion of cellular prion protein and other brain proteins. Apparent resistance of cellular prion protein to proteolysis by PK appears to be directly attributable to the inhibition of PK activity by copper-(II) ions	Tritirachium album Limber

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	at equilibrium two to three copper ions bind stoichiometrically to PK and destroy its activity. Initial reversible and weak binding phase and a slower, irreversible abolition of activity with a half-time of 6 min at saturating copper ion concentrations. PK digestion of cellular prion proteins and other proteins in brain homogenate is inhibited in a concentration-dependent manner at concentrations of more than 1 mM. Presence of calcium ions, up to 10 mM, has no effect on copper inhibition	Tritirachium album Limber
additional information	not inhibited by Zn2+ or Mn2+	Tritirachium album Limber

Organism

Organism	UniProt	Comment	Textmining
Tritirachium album Limber	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	PK is very effective in destroying cellular prion proteins and endogenous proteases present in brain homogenate	Tritirachium album Limber	?	-	?
p-nitrophenyl acetate + H2O	-	Tritirachium album Limber	p-nitrophenol + acetate	-	?

Synonyms

Synonyms	Comment	Organism
Proteinase K	-	Tritirachium album Limber

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.7	7.4	-	Tritirachium album Limber

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)