Cloned (Comment) | Organism |
---|---|
gene SBT1.9 or At5g67090, recombinant expression of soluble mature enzyme AtSBT1.9 fused to maltose-binding protein lacking the signal peptide in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme contains a signal peptide | Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FHA4 | - |
- |
Arabidopsis thaliana Col-0 | Q9FHA4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant mature enzyme AtSBT1.9 fused to maltose-binding protein from Escherichia coli BL21(DE3) by amylose affinity chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.23 | - |
purified recombinant MBP-tagged mature enzyme, pH 7.0, 25°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
azocasein + H2O | - |
Arabidopsis thaliana Col-0 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 117500, recombinant soluble mature enzyme AtSBT1.9 fused to maltose-binding protein, SDS-PAGE, x * 77500, about, mature untagged enzyme, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtSBT1.9 | - |
Arabidopsis thaliana |
subtilase | - |
Arabidopsis thaliana |
subtilase subfamily 1 member 9 | - |
Arabidopsis thaliana |
subtilisin-like serine protease | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | 70 | recombinant enzyme, over 50% of maximal activity within the range of 18-50°C, 40% of maximal activity at 60°C, 10% at 70°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | recombinant enzyme, over 40% of maximal activity within this range | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the Arabidopsis thaliana genome encodes 56 subtilisin-like serine proteases (subtilases), enzyme AtSBT1.9 is a member of the subtilase subfamily 1 | Arabidopsis thaliana |