Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged wild-type and mutant S684A Lon in Escherichia coli strain BLR-DE3 | Azorhizobium caulinodans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout mutant generation by lon gene deletion, generation of a lon/reb double knockout mutant | Azorhizobium caulinodans |
S684A | site-directed mutagenesis, catalytic site mutant | Azorhizobium caulinodans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Azorhizobium caulinodans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azorhizobium caulinodans | A8HYF7 | gene lon | - |
Azorhizobium caulinodans ORS 571 | A8HYF7 | gene lon | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant S684A Lon from Escherichia coli strain BLR-DE3 by nickel affinity chromatography | Azorhizobium caulinodans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | two kinds of host cells are observed in the stem nodules of host legume Sesbania rostrata | Azorhizobium caulinodans | - |
Synonyms | Comment | Organism |
---|---|---|
lon protease | - |
Azorhizobium caulinodans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Azorhizobium caulinodans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Azorhizobium caulinodans |
General Information | Comment | Organism |
---|---|---|
malfunction | the stem nodules in the host legume Sesbania rostrata formed by the lon mutant show little or no nitrogen fixation activity. The reb genes are highly expressed in the lon mutant, high expression of reb genes in part causes aberrance in the Azorhizobium caulinodans-Sesbania rostrata symbiosis | Azorhizobium caulinodans |
physiological function | Lon protease is required to suppress the expression of the reb genes. Lon protease is also involved in the regulation of exopolysaccharide production and autoagglutination of bacterial cells | Azorhizobium caulinodans |