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Literature summary for 3.4.21.46 extracted from

  • Ding, M.; Fan, J.; Wang, W.; Wang, H.; Liu, H.
    Molecular characterization, expression and antimicrobial activity of complement factor D in Megalobrama amblycephala (2019), Fish Shellfish Immunol., 89, 43-51 .
    View publication on PubMed
Search for more literature for 3.4.21.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Megalobrama amblycephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement factor B + H2O Megalobrama amblycephala
-
 fragment Ba + fragment Bb
-
 ?

Organism

Organism UniProt Comment Textmining
Megalobrama amblycephala
-
 blunt snout bream
-

Purification (Commentary)

Purification (Comment) Organism
Ni-agarose column chromatography Megalobrama amblycephala

Source Tissue

Source Tissue Comment Organism Textmining
blood low expression Megalobrama amblycephala
-
brain moderate expression Megalobrama amblycephala
-
gill moderate expression Megalobrama amblycephala
-
head kidney
-
 Megalobrama amblycephala
-
heart
-
 Megalobrama amblycephala
-
intestine low expression Megalobrama amblycephala
-
kidney highest expression in the kidney in healthy adults Megalobrama amblycephala
-
liver
-
 Megalobrama amblycephala
-
muscle moderate expression Megalobrama amblycephala
-
spleen moderate expression Megalobrama amblycephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement factor B + H2O
-
 Megalobrama amblycephala fragment Ba + fragment Bb
-
 ?

Subunits

Subunits Comment Organism
? x * 27200, calculated from amino acid sequence Megalobrama amblycephala

pI Value

Organism Comment pI Value Maximum pI Value
Megalobrama amblycephala calculated from amino acid sequence
-
 6.7

Expression

Organism Comment Expression
Megalobrama amblycephala after challenged with Aeromonas hydrophila, the enzyme, at both mRNA and protein levels, is upregulated in the kidney, spleen, liver and head-kidney up

General Information

General Information Comment Organism
physiological function the enzyme shows antimicrobial activity against both gram-positive bacteria like Staphylococcus aureus and gram-negative bacteria like Aeromonas hydrophila Megalobrama amblycephala