Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | - |
Sitophilus zeamais | |
benzamidine | - |
Sitophilus zeamais | |
Bowman-Birk inhibitor | - |
Sitophilus zeamais | |
E64 | weak inhibition | Sitophilus zeamais | |
leupeptin | - |
Sitophilus zeamais | |
N-alpha-tosyl-L-lysine chloromethyl ketone | - |
Sitophilus zeamais | |
pepstatin | - |
Sitophilus zeamais | |
phenylmethyl sulfonyl fluoride | - |
Sitophilus zeamais | |
Soybean trypsin inhibitor | - |
Sitophilus zeamais |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Sitophilus zeamais |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, best at 20 mM | Sitophilus zeamais |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sitophilus zeamais | - |
insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity | - |
Purification (Comment) | Organism |
---|---|
native enzymes partially by aprotinin affinity chromatography, 51.2fold | Sitophilus zeamais |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
529 | 875 | partially purified native trypsin-like serine proteinases, pH 8.2, 25°C | Sitophilus zeamais |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Sitophilus zeamais | ? | - |
? | |
N-alpha-p-tosyl-L-Arg methyl ester + H2O | - |
Sitophilus zeamais | N-alpha-p-tosyl-L-Arg + methanol | - |
? | |
N-benzoyl-L-arginine 4-nitroanilide + H2O | the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains | Sitophilus zeamais | N-benzoyl-L-arginine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypsin-like serine proteinase | - |
Sitophilus zeamais |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Sitophilus zeamais |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | assay range | Sitophilus zeamais |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Sitophilus zeamais |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | assay range | Sitophilus zeamais |
General Information | Comment | Organism |
---|---|---|
additional information | enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains | Sitophilus zeamais |