Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Na+ | wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
A171S/Y172S/G173S/N174F/E175I/E180M/G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y | contrary to wild-type, mutant may be activated by Na+, mutations in 170 loop increase susceptibility to Na+ further | Streptomyces griseus |
G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y | twofold increase in KM-value | Streptomyces griseus |
Y172M/G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y | contrary to wild-type, mutant may be activated by Na+ | Streptomyces griseus |
Y172S/G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y | contrary to wild-type, mutant may be activated by Na+ | Streptomyces griseus |
Y172S/Y217E/P222K/Y224K/P225Y | 30fold increase in KM-value, contrary to wild-type, mutant may be activated by Na+ | Streptomyces griseus |
Y217E/P222K/Y224K/P225Y | twofold increase in KM-value | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0089 | - |
factor Xa | wild-type, pH 8.0, 25°C | Streptomyces griseus | |
0.0205 | - |
factor Xa | mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
0.0222 | - |
factor Xa | mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
0.285 | - |
factor Xa | mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | P00775 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor Xa + H2O | - |
Streptomyces griseus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
factor Xa | mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
9.25 | - |
factor Xa | mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
77.7 | - |
factor Xa | mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
86.4 | - |
factor Xa | mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C | Streptomyces griseus | |
124 | - |
factor Xa | wild-type, pH 8.0, 25°C | Streptomyces griseus |